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Protective effect of saturated hydrogen saline on hyperoxic lung injury

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
Issue:
3
DOI:
10.3760/cma.j.issn.1009-6906.2011.03.001
Key Word:
氢气;急性肺损伤;氧化应激;氧中毒;Hydrogen;Acute lung injury;Oxidative stress;Hyperoxia

Abstract: Objective To investigate the protective effect of saturated hydrogen saline on acute lung injury induced by hyperoxia in rats.Methods Forty-eight male SD rats were randomly divided into 4 groups:the normal air control group,the physiological saline group,the saturated hydrogen saline group and the saturated nitrogen saline group. With the exception of the control group, the animals in the other groups exposed continuously to pure medical oxygen (O2 concentration>98%) for 60 h to develop the chronic oxygen poisoning model. Then, the animals were treated with the physiological saline, the saturated hydrogen saline and the saturated nitrogen saline in the process of oxygen exposure. Severity of acute lung injury induced by hyperoxia (HALI) was assessed by the volume of pleural effusion, water content and Hematoxylin and Eosin (HE) staining. Malondialdehyde (MDA) and the expression of superoxidase dismutase (SOD) were monitored for assessment of oxidative stress, and changes in DNA oxidation product, 8-hydroxy-desoxyguanosine (8-OHDG) were also monitored.Results When a comparison was made with the physiological saline group,saturated hydrogen saline treatment could significantly relieve lung injury induced by chronic pure oxygen exposure as indicated by HE staining, decrease volume of pleural effusion and pulmonary edema. Moreover, saturated hydrogen saline treatment could inhibit lipid and DNA oxidation. Experiments showed that saturated nitrogen saline treatment had no protective effect on hyperoxic lung injury.Conclusions Saturated hydrogen saline treatment could alleviate lung injury induced by hyperoxia.

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