You Position: Home > Paper
The effect of ultraviolet on cytokine secretion in human keratinocyte HaCaT cell line
( views:178, downloads:4 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1009-6906.2002.02.001
- Key Word:
- 紫外线;HaCaT细胞;细胞因子
Abstract： 目的观察紫外线(ultraviolet,UV)对体外培养的角质形成细胞株(HaCaT细胞)细胞因子分泌的影响.方法 UV照射佛泊脂和脂多糖(PMA/LPS)刺激的HaCaT细胞,用ELISA法检测细胞培养上清液中细胞因子IL-1α、IL-2、IL-6和IL-8的含量.结果正常HaCaT细胞有IL-1α、IL-2、IL-6和IL-8的自发性分泌.PMA和LPS刺激HaCaT细胞,其IL-1α分泌量下降(P＜0.05),IL-6和IL-8分泌量明显增加(P＜0.01).正常HaCaT细胞经UV照射,IL-1α、IL-2、IL-6的分泌无明显变化(P＞0.05),IL-8分泌量升高(P＜0.05).PMA和LPS刺激的HaCaT细胞经UV照射,其IL-1α、IL-6和IL-8的分泌量明显增加(P＜0.01).结论 UV对不同状态下HaCaT细胞的作用是不同的.
- [1]张建中,马圣清,丸山幸治,等.1,25-双羟维生素D_3和MC903对培养人角朊细胞白介素1α及白介素8分泌的影响[J].1996,(2).
- [2]Jean Krutmann,Akimichi Morita.Mechanisms of Ultraviolet (UV) B and UVA Phototherapy[J].1999,4(1).
- [3]Jimbow K,Kondo S.Dose-dependent induction of IL-12 but not IL-10 from human keratinocytes after exposure to ultraviolet light A.[J].1998,177(3).
- [4]L. Kemény,G. Michel,A. Dobozy,etc.Cytokine system as potential target for antipsoriatic therapy[J].1994,3(1).
- [5]C D, Enk,D, Sredni,A, Blauvelt,etc.Induction of IL-10 gene expression in human keratinocytes by UVB exposure in vivo and in vitro.[J].1995,154(9).
- [6]M, Stein,A, Bernd,A, Ramirez-Bosca,etc.Measurement of anti-inflammatory effects of glucocorticoids on human keratinocytes in vitro. Comparison of normal human keratinocytes with the keratinocyte cell line HaCaT.[J].1997,47.
- [7]J D, Bos,M L, Kapsenberg.The skin immune system: progress in cutaneous biology.[J].1993,14(2).