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Effect of hyperbaric oxygen on mitochondrial membrane potential and apoptosis of cells in rats after carbon monoxide poisoning
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1009-6906.2001.02.014
- Key Word:
- 高压氧;一氧化碳中毒;线粒体膜电位;凋亡
Abstract： 目的观察一氧化碳中毒(COP)后脑损伤时线粒体膜电位及细胞凋亡数量的变化以及高压氧(HBO)对其影响.方法 Wistar大鼠共128只,采用配伍组设计,按随机抽样原则将动物分成4组16小组.正常对照组8只;CO组COP后第1,5,10,15,20天各8只;CO+HP组高气压处理后第1,5,10,15,20天各8只;CO+HBO组HBO处理后第1,5,10,15,20天各8只.用流式细胞仪测定COP大鼠在不同时间段海马神经细胞线粒体膜电位及海马神经细胞凋亡细胞相对百分比(%)的变化和HBO治疗后的改变.结果 CO组在COP后大鼠海马神经细胞线粒体膜电位在第1,5,10天降低(P＜0.01～0.05);CO+HBO组在第1,5天降低(P＜0.01～0.05).CO组COP后大鼠海马神经细胞凋亡细胞相对百分比在第1,5,10,15,20天高于对照组(P＜0.01);而CO+HBO组只在第1,5,10天高于对照组(P＜0.01).结论 COP后大鼠海马神经细胞线粒体膜电位降低,凋亡细胞数增加,并持续很长时间.而HBO处理可缩短线粒体膜电位降低的时间和减少凋亡细胞数百分比.
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