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Expression of homo sapiens eukaryotic translation elongation factor 1 alpha 2 in pancreatic carcinoma
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1935.2007.01.004
- Key Word:
- 胰腺肿瘤;延长因子;实时定量PCR;免疫组织化学
Abstract： 目的 研究人类真核翻译延长因子1A2(EEF1A2)及其表达产物eEF1A2蛋白在人胰腺癌细胞株和组织标本中的表达.方法 收集人胰腺导管腺癌病理标本15例,人正常胰腺组织标本8例.运用RT-PCR、Realtime-PCR、Western blot以及免疫组化等技术检测人胰腺癌细胞株SW1990、BxPC3、PaTu8988和胰腺癌组织标本中EEF1A2的表达水平.结果 人胰腺腺癌细胞株PaTu8988、SW1990和BxPC3中,EEF1A2 mRNA和eEF1A2蛋白表达水平与正常胰腺组织相比,均明显上调.8例癌旁正常胰腺组织标本中仅1例胰腺导管细胞内eEF1A2呈弱阳性表达,而15例胰腺导管腺癌病理标本中,胰腺导管腺癌细胞内eEF1A2均呈中至强阳性表达,两者比较有显著差异(P ＜ 0.05).结论 EEF1A2在胰腺癌中呈异常高表达.EEF1A2的致癌机制可能与加速蛋白质翻译速度、抑制凋亡以及改变细胞骨架有关.EEF1A2有可能成为胰腺癌一个有效的诊断标志物和基因治疗靶点.
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