You Position: Home > Paper
Clinical significance of APE expression in pancreatic carcinoma
( views:225, downloads:4 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1674-1935.2006.02.003
- Key Word:
- 胰腺肿瘤;APE/ref-1(无嘌呤嘧啶核酸内切酶);免疫组织化学
Abstract： 目的研究胰腺癌组织APE的表达及其与临床肿瘤指标的关系.方法 (1)通过逆转录-聚合酶链反应(RT-PCR)方法检测4株胰腺癌细胞株APE mRNA的表达;(2)收集37例手术切除的胰腺癌及12例相应癌旁胰腺组织石蜡标本,应用EnVision免疫组化法分别检测癌组织和癌旁胰腺组织APE的表达情况.结果 4株胰腺癌细胞株均有APE mRNA表达;37例胰腺癌中APE阳性表达33例(89.2%),其中APE胞核阳性表达7例(21.2%),胞质阳性表达5例(15.2%),胞质及胞核均表达21例(63.6%).12例癌旁胰腺组织导管及腺泡呈阴性表达,胰岛胞质呈阳性表达,胰腺上皮内瘤变呈胞核阳性表达.APE阳性率与肿瘤分化程度,肿瘤大小、淋巴及远处转移无关(P ＞ 0.1).结论 APE在胰腺癌组织中有较高的阳性表达率,检测其定位的特异性表达可能有助于胰腺癌的早期诊断.
- [1]Pucillo C,Tell G,Puglisi F,etc.TSH controls Ref-1 nuclear translocation in thyroid cells.[J].2000,24(3).
- [2]G, Fritz.Human APE/Ref-1 protein.[J].2000,32(9).
- [3]Demple B,Harrison L.Repair of Oxidative Damage to DNA: Enzymology and Biology[J].1994.
- [4]Pucillo C,Pellizzari L,Tell G,etc.Redox potential controls the structure and DNA binding activity of the paired domain.[J].1998,273(39).
- [5]B, Thomson,R, Tritt,M, Davis,etc.Histology-specific expression of a DNA repair protein in pediatric rhabdomyosarcomas.[J].2001,23.