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Expression of mesothelin in pancreatic carcinoma tissue and its clinical significance
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1935.2005.01.005
- Key Word:
- 胰腺肿瘤;Mesothelin;免疫组织化学
Abstract： 目的研究胰腺癌组织mesothelin的表达及其与临床肿瘤指标的关系.方法 (1)通过逆转录-聚合酶链反应(RT-PCR)方法检测5株胰腺癌细胞株mesothelin mRNA的表达;(2)收集54例手术切除的胰腺癌及42例相应癌旁胰腺组织石蜡标本,利用EnVision免疫组化法分别检测癌组织和癌旁胰腺组织Mesothelin的表达情况.结果 5株胰腺癌细胞株均有mesothelin mRNA表达;54例胰腺癌中mesothelin阳性表达41例(75.9%),2例癌旁胰腺组织中均无阳性表达;mesothelin阳性率与肿瘤分化程度相关(P ＜ 0.005),与肿瘤大小、淋巴及远处转移无关(P ＞ 0.05).结论 Mesothelin在胰腺癌组织中有较高的阳性表达率,检测其表达有助于胰腺癌的诊断,并可作为判断胰腺癌恶性程度的重要指标.
- [1]Taniguchi Y,Tamura M,Ochi N,etc.Molecular cloning and expression of megakaryocyte potentiating factor cDNA.[J].1995,270(37).
- [2]Beers R,Pastan I,Chowdhury PS,etc.Isolation of a high-affinity stable single-chain Fv specific for mesothelin from DNA-immunized mice by phage display and construction of a recombinant immunotoxin with anti-tumor activity.[J].1998,95(2).
- [3]Ira Pastan,Kai Chang.Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers[J].1996,93(1).
- [4]Fu N,Ye Z,Yang Y,etc.Soluble member(s) of the mesothelin/megakaryocyte potentiating factor family are detectable in sera from patients with ovarian carcinoma.[J].1999,96(20).
- [5]Hassan Raffit,Viner Jaye L,Wang Qing-Cheng,etc.Anti-Tumor Activity of K1-LysPE38QQR, an Immunotoxin Targeting Mesothelin, a Cell-Surface Antigen Overexpressed in Ovarian Cancer and Malignant Mesothelioma[J].2000.
- [6]Bera TK,Pastan I.Mesothelin is not required for normal mouse development or reproduction.[J].2000,20(8).