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Inhibition effect of recombinant adenovirus mediated antisense VEGF on the growth of pacreatic carcinoma cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1935.2002.01.005
- Key Word:
- 反义血管内皮生长因子;腺病毒;胰腺癌;基因治疗
Abstract： 目的构建表达反义VEGF165的重组腺病毒,探讨腺病毒介导的VEGF165反义核酸治疗胰腺癌的可行性.方法应用基因重组技术,将513 bp的人VEGF1 65 cDNA反向克隆到腺病毒粘粒载体pAxCAwt的Swa I酶切位点.重组粘粒与腺病毒DNA末端肽复合物混合后以磷酸钙共沉淀法转染293细胞,制备出表达反义VEGF165的重组腺病毒.建立胰腺癌裸鼠皮下种植瘤模型,瘤体内直接注射重组腺病毒,观察肿瘤的生长及血管生成情况.结果成功构建了反义VEGF165腺病毒粘粒载体pAxCAwt-αVEGF,并制备出高滴度的反义VEGF165重组腺病毒,注入胰腺癌瘤体内后,治疗组肿瘤生长速度比对照组明显减慢(P＜0.01),治疗组肿瘤微血管密度也明显减少(P＜0.01).结论反义VEGF165重组腺病毒可以抑制胰腺癌的血管生成和肿瘤生长,有潜在的应用价值.
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