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Expression and significance of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in adenomyosis

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
Issue:
6
DOI:
10.3760/cma.j.issn.1674-1927.2010.06.003
Key Word:
基质金属蛋白酶2;基质金属蛋白酶抑制因子2;子宫内膜;子宫腺肌病;反转录聚合酶链反应;Matrix metalloproteinase-2;Tissue inhibitor of metalloproteinase-2;Endometrium;Adenomyosis;Reverse transcriptase polymerase chain reaction

Abstract: Objective To investigate the expression and significance of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in ectopic and eutopic endometrium in adenomyosis. Methods Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the mRNA of MMP-2 and TIMP-2 in ectopic and eutopic endometrium from 42 patients who received panhysterectomy for adenomyosis (AM ectopic group and AM eutopic group), and in eutopic endometrium from 32 who received panhysterectomy for uterine myoma (normal control group), in The First Municipal People's Hospital of Guangzhou from February 2006 to September 2007. Results ①The relative expression level of MMP-2 mRNA in AM ectopic endometrium group (1.43±0.32) was significantly higher than that in normal group (1.22±0.35) and AM eutopic group (0.97±0.37) (P<0.05), whereas the relative expression level of TIMP-2 mRNA in both AM ectopic and eutopic groups (0.96±0.31 and 1.13±0.30) was significantly lower than that in the normal control group (1.29±0.19, P<0.05). ②The expression of MMP-2 and TIMP-2 showed phase-dependent changes in the eutopic endometrium, with higher level in secretory phase than in proliferative phase (P<0.05). However, in ectopic endometrium, the expression was statistically similar in both phases (P>0.05). ③The highest ratio of MMP-2/TIMP-2 was in ectopic endometrium, and the lowest in normal endometrium, with significant difference comparing the AM ectopic group to AM eutopic group and normal control group. Conclusion High expression of MMP-2 and low expression of TIMP-2 are found in ectopic and eutopic endometrium from AM patients. The MMP-2/TIMP-2 dysequilibrium in ectopic endometrium may underlie its enhanced activity for invasion and degradation of extracellular matrix.

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