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Proliferation Inhibition and Apoptosis Induction of U937 Cells by Quercetin
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1008-634X.2006.02.003
- Key Word:
- 槲皮素;U937;凋亡;细胞周期
Abstract： 目的探讨黄酮类化合物槲皮素(Que)对人类单核细胞白血病U937细胞系的抑制增殖和诱导凋亡的作用.方法应用MTT法检测不同浓度槲皮素对U937细胞的增殖抑制作用;AO/PI荧光染色后倒置荧光显微镜下观察细胞形态学变化;琼脂糖凝胶电泳测定细胞DNA的片段化;应用流式细胞仪检测细胞凋亡率及细胞周期分布.结果槲皮素能明显抑制U937细胞增殖,并存在剂量-效应关系和时间-效应关系;诱导U937细胞出现凋亡所具有的形态学和生化特征;随着槲皮素浓度升高,凋亡细胞和坏死细胞比例增加;将细胞特异性地阻滞在S期,出现凋亡峰.结论槲皮素能抑制U937细胞增殖,诱导细胞凋亡,并具有细胞周期特异性.
- [1]王艳芳,王新华,朱宇同.槲皮素药理作用研究进展[J].2003,(2).
- [2]钟璐,陈芳源,韩洁英,等.槲皮素对NB4,HL-60细胞形态、PML mRNA及蛋白表达的影响[J].2001,(1).
- [3]赖永洪,翟莺莺,叶铁真.槲皮素对柔红霉素所致心肌损伤的保护作用[J].2005,(1).
- [4]崔巍.流式细胞术PI染色进行细胞周期分析的原理是什么?[J].2004,(3).
- [5]主编章静波.组织和细胞培养技术[M].2002.
- [6]Csokay B.,Weber G.,Olah E.,etc.Molecular Mechanisms in the Antiproliferative Action of Quercetin[J].1997,60(24).
- [7]C J, Kemp,S, Sun,K E, Gurley.P53 Induction and Apoptosis in Response to Radio- and Chemotherapy in Vivo is Tumor-Type-Dependent.[J].2001,61(1).
- [8]J, Guzy,J, Kusnír,M, Mareková,etc.Effect of quercetin on daunorubicin-induced heart mitochondria changes in rats.[J].2003,52(6).
- [9]Novotny L,Rauko P,Cipakova I,etc.Effects of flavonoids on cisplatin-induced apoptosis of HL-60 and L1210 leukemia cells.[J].2003,27(1).
- [10]编著胡野, 凌志强, 单小云.细胞凋亡的分子医学[M].2002.
- [11]T B, Kang,N C, Liang.Studies on the inhibitory effects of quercetin on the growth of HL-60 leukemia cells.[J].1997,54(9).