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Analysis of the Immunoglobulin Heavy Chain Variable Region gene Fingerprinting of Human Neonates
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1008-634X.2004.01.004
- Key Word:
- 新生儿;免疫球蛋白;重链可变区;基因;指纹图谱
Abstract： 目的构建不同胎龄新生儿IgH基因指纹图谱,探讨新生儿成熟度对IgH基因谱型多样性的影响.方法提取27～42周新生儿RNA、进行RT-PCR反应、6%变性聚丙烯酰胺凝胶电泳和银染显色,获得不同胎龄新生儿IgH基因指纹图谱,用Tiger凝胶图像分析仪进行曲线转化和分析.结果各家族指纹图谱转化后的曲线下面积积分值,在28～32周、33～36周和37～42周新生儿之间无差异;但胎龄27周新生儿曲线下面积小于胎龄28～42周新生儿.结论 28周可能是人类IgH基因谱型发育由不完善到趋于完善的一个转折点,28周到足月的新生儿IgH基因谱型发育趋于稳定.
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