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Establishment of Rat Model of Brain Cold Injury and Study on Bcl-2 Protein Expression
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1674-1927.2001.05.005
- Key Word:
- 大鼠;冷冻;脑损伤;Bcl-2基因;凋亡
Abstract： 目的建立大鼠脑冷冻伤模型并探讨脑组织细胞冷冻伤中Bcl-2的表达,提供抗损伤治疗的新思路. 方法运用液氮冷冻器作用于大鼠左侧顶部脑组织后2h、6h、12h,建立脑冷冻伤模型,同时观察冷冻伤侧脑组织病理形态学改变与Bcl-2表达的变化. 结果液氮作用后2h,脑组织已出现充血、水肿及神经元坏死的表现,并于12h明显累及海马回神经元.有价值的是,与坏死交界处的健在神经元呈现了Bcl-2的高表达. 结论说明了脑组织中神经元的损伤与Bcl-2基因蛋白的表达有关,提示通过抗细胞凋亡的途径对脑损伤治疗具有应用前景.
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