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Analysis of the Clonal Expansion of TCR Vβ Subfamily Genes and T Cell Clonality in AML-M2a Using Genescan
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENG INEERING
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1674-1927.2001.04.002
- Key Word:
- T细胞受体;Vβ亚家族;克隆性;急性髓系白血病;基因扫描
Abstract： 目的了解AML-M2a患者外周血中TCR Vβ亚家族T细胞的分布和克隆性情况.方法利用RT-PCR扩增14例AML-M2a患者外周血单个核细胞中24个TCR Vβ亚家族基因的CDR3,了解TCR Vβ亚家族T细胞的分布.阳性产物进一步经基因扫描分析了解其克隆性.结果 14例AML-M2a患者选择性表达3～10个Vβ亚家族,其中部分Vβ亚家族呈寡克隆增殖.结论 AML-M2a患者TCR Vβ亚家族T细胞的倾斜性分布和克隆性增殖,可能是机体对M2a细胞刺激所产生的特异性免疫反应.
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