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Expression of BCL-2 in MCF-7 Cells:a Mechanism for Apoptosis Antagonism and Immune Evasion in Breast Cancer Cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1674-1927.2000.04.003
- Key Word:
- 乳腺癌;BCL-2;反义寡核苷酸;肿瘤浸润淋巴细胞
Abstract： 目的观察乳腺癌细胞株BCL-2基因表达与其耐受肿瘤浸润淋巴细胞(TIL)免疫攻击的关系,探索应用BCL-2硫代反义寡核苷酸(BCL-2SON)治疗乳腺癌的途径.方法BCL-2硫代反义寡核苷酸处理乳腺癌细胞株MCF-7,Western印迹法观察其对BCL-2表达的抑制;并将MCF-7细胞与乳腺癌标本分离出来的TIL共同培养,通过JAM试验观察TIL诱导MCF-7细胞凋亡的情况.结果BCL-2SON处理的MCF-7细胞无BCL-2表达,并在JAM试验中随着TIL浓度增加,凋亡细胞也增加;未经处理的对照组可见BCL-2表达,JAM试验中随着TIL浓度增加,凋亡细胞的量无明显变化.结论BCL-2表达的乳腺癌细胞可对抗肿瘤浸润淋巴细胞的免疫攻击,BCL-2SON可作为治疗乳腺癌的新途径.
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