Abstract： A case of human papillomarivru type (HPV) 7-associated warts in toe webs and perianal sites is reported. The 35-year-old patient, who was an office worker in a company, presented with soft, friable, vegetating clusters in toe webs and perianal sites for one year. Histopathological examination of warts in toe webs revealed parakeratosis, mild hyperkeratesis, papillomatous hyperplasia, acanthosis in epidermis as well as vasodilatation and perivascular lymphoid infiltrates in dermis. There were numerous vacuolated cells in stratum granulosum and upper stratum spinosum which were lager than normal cells with condensed nuclei and perinuclear halo. DNA was extracted from samples taken from toe webs and perianal sites. PCR was performed using consensus primers, i.e. MY and GP, to amplify HPV conservative region followed by sequencing of the amplicons. Gene sequencing showed that both samples were positive for HPV7. And species-specific PCR revealed that these samples were negative for HPV6, 11, 16 or 18, but positive for HPV 7, which was consistent with the results of gene sequencing.