Effect of TRAIL on NB4 and K562 cell lines and its relationship with TRAIL receptors expression

( views:1386, downloads:0 )
ZHANG Ying-chun(Department of Pathology, Xuzhou Medical College, Xuzhou 221002, China)
WANG Wei-ya()
YU Jian-bo()
LIU Wei-ping()
Journal Title:
Journal of Leukemia & Lymphoma
Volume 21, Issue 10, 2012
Key Word:
NB4 cells;K562 cells;Tumour necrosis factor related apoptosis-inducing ligand;Death receptor;Decoy receptors

Abstract: Objective To investigate the effect of TRAIL on NB4 and K562 cell lines, and its relationship with TRAIL releptors.Methods Jurkat cells were used as positive control,NB4 and K562 cells were treated with different concentrations of TRAIL. Cell morphologic changes were monitored. The cell proliferation was evaluated by MTT assay. The expression of TRAIL receptor were determined by flow cytometry.Results MTT assay showed that TRAIL inhibited the growth of NB4 and Jurkat cells in vitro in a dose-and time-dependent manner,but the effect of TRAIL on Jurkat cells was stronger than that on NB4 cells.However, the growth of K562 was not inhibited. Flow cytometry analysis revealed that DR4,DR5 and DcR1 were expressed higher in NB4 and K562 cells, but the levels of DR4 and DcR1 were very low in K562 cells.DR5 was expressed in Jurakat cells with low level. No DcR2 was detected on the surface of all the three cell lines.Conclusion NB4 cell line is moderately sensitive to TRAIL,and K562 cell line is resistant to TRAIL.The sensitivity of NB4 cells to TRAIL may be associated with the expression of DcR1, but the sensitivity of K562 cells have nothing to do with the expression of TRAIL receptors.

  • [1]Szegezdi E,Reis CR,van der Sloot AM,et al.Targeting AML through DR4 with a novel variant of rhTRAIL. J Cell Mol Med, 2011, 15:2216-2231.
  • [2]Walczak H,Miller RE,Ariail K,et al.Tumoricidal activity of tumor necrosis factor-related apoptosis-inducing ligand in vivo. Nat Med,1999,5:157-163.
  • [3]Ashkenazi A. Targeting death and decoy receptors of the tumournecrosis factor superfamily.Nat Rev Cancer,2002,2:420-430.
  • [4]Thorburn A, Behbakht K, Ford H. TRAIL receptor-targeted therapeutics: resistance mechanisms and strategies to avoid them.Drug Resist Updat,2008,11:17-24.
  • [5]Testa U. TRAIL/TRAIL-R in hematologic malignancies. J Cell Biochem,2010,110:21-34.
  • [6]Weng C,Li Y,Xu D,et al.Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells.J Biol Chem,2005,280:10491-10500.
  • [7]Du YW,Chen JG,Bai HL,et al.A novel agonistic anti-human death receptor 5 monoclonal antibody with tumoricidal activity induces caspase-and mitochondrial-dependent apoptosis in human leukemia Jurkat cells.Cancer Biother Radiopharm,2011,26:143-152.
  • [8]Altucci L,Rossin A,Raffelsberger W,et al.Retinoic acid-induced apoptosis in leukemia cells is mediated by paracrine action of tumorselective death ligand TRAIL.Nat Med,2001,7:680-680.
  • [9]Park SJ,Kim M J,Kim HB,et al.Cotreatment with apicidin overcomes TRAIL resistance via inhibition of Bcr-Abl signaling pathway in K562 leukemia cells.Exp Cell Res,2009,315:1809-1818.
  • [10]郝继辉,郝希山,俞鸣,等.肿瘤坏死因子凋亡诱导配体诱导白血病细胞凋亡中抵抗机制的初步探讨.中华血液学杂志,2006,27:379-382.
  • [11]Zhang L, Fang B. Mechanisms of resistance to TRAIL-induced apoptosis in cancer.Cancer Gene Ther,2005,12:228-237.
  • [12]Merino D, Lalaoui N, Morizot A, et al. Differential inhibition of TRAIL-mediated DR5-DISC formation by decoy receptors 1 and 2.Mol Cell Biol,2006,26:7046-7055.
  • [13]Jin Z,McDonald ER 3rd,Dicker DT,et al.Deficient tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) death receptor transport to the cell surface in human colon cancer cells selected for resistance to TRAIL-induced apoptosis. J Biol Chem, 2004, 279:35829-35839.
  • [14]Zhang Y,Yoshida T,Zhang B.TRAIL induces endocytosis of its death receptors in MDA-MB-231 breast cancer cells. Cancer Biol Ther,2009,8:917-922.
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn