Significance and expression of PTEN, MLL gene in T lymphoblastic lymphoma/leukemia
- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-9921.2011.04.004
- Key Word:
- 淋巴瘤/白血病,淋巴母细胞;PTEN,基因,肿瘤抑制;基因,MLL;Lymphoma/leukaemia,lymphoblastic;PTEN,gene,tumor suppressor;Gene,MLL
Abstract： Objective To investigate the significance and expression of PTEN, MLL in T lymphoblastic lymphoma/leukaemia(T-LBL/ALL). Methods Seventy-six cases of T-LBL/ALL were studied by using immunohistochemical EnVision method for PTEN. Fluorescence in-situ hybridization (FISH) for MLL gene (located on chromosome 11q23) was performed to detect its breakage and amplification. Results Among the 76 cases ofT- LBL/ALL, the positive rate of PTEN was 64.47 % (49/76), lower than that in reactivated lymphoid tissue (100 %, 20/20) (λ2= 19.220, P ＜0.05). PTEN expression was reversely correlated to theclinical stage, Ki-67 index and LDH level (P ＜0.05). Among the 76 cases, MLL gene with breakage of 11q23 was detected in 13 cases (17.11%), and amplification in 18 cases (23.68 %). Survival rate ot MLL gene breakage group was lower than that of non-breakage group (25.0 %, 43.6 %). Survival rate of MLL gene amplification group was lower than that of non-amplification group too (17.1%, 42.7 %). Both of breakage and amplification were related to prognosis ( λ 2 = 11.357, λ 2 = 4.533; P ＜0.05). Conclusion Anti-oncogene PTEN down-regulation may play an important role on the development and proceeding of T-LBL/ALL. MLL gene with breakage and amplification of 11q23 are helpful to predict prognosis of T-LBL/ALL. The case with MLL gene breakage and amplification of T-LBL/ALL may have a poor prognosis. It hints this group maybe a subtype of T-LBL/ALL.
- [1]孙宏伟,郑华川,吴东瑛,等.PTEN蛋白和Caspase-3表达在胃原发恶性淋巴瘤的意义[J].2004,(3).
- [2]李祥勇,林观平,周克元.转染抑癌基因PTEN对人乳腺癌ZR-75-1细胞的细胞周期和增殖能力的影响[J].2006,(2).
- [3]赵喜晨,李承文,代芸,等.联合间期和中期荧光原位杂交确定成人急性白血病患者11q23/MLL异常[J].2006,(10).
- [4]尹亮,李锋.抑癌基因PTEN与软组织肉瘤[J].2009,(2).
- [5]张丽君,卢香兰,何娟,等.急性髓性白血病混合谱系白血病基因重排的研究[J].2006,(32).
- [6]T, Sano,H, Lin,X, Chen,etc.Differential expression of MMAC/PTEN in glioblastoma multiforme: relationship to localization and prognosis.[J].1999,59.
- [7]Han X,Bueso-Ramos CE.Precursor T-cell acute lymphoblastic leukemia/lymphoblastic lymphoma and acute biphenotypic leukemias.[J].2007,127(4).
- [8]Dominguez M,Palomero T,Ferrando AA.The role of the PTEN/AKT Pathway in NOTCH1-induced leukemia.[J].2008,7(8).
- [9]Marynen P,Cools J,De Keersmaecker K.Genetic insights in the pathogenesis of T-cell acute lymphoblastic leukemia.[J].2005,90(8).
- [10]Michaux L,Cools J,Hagemeijer A,etc.Cytogenetics and molecular genetics of T-cell acute lymphoblastic leukemia: from thymocyte to lymphoblast.[J].2006,20(9).
- [11]Li, Jing,Yen, Clifford.PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate canc[J].1997,275(5308).
- [12]Ueki T,Kawamura N,Koyama M,etc.PTEN/MMAC1 mutations in hepatocellular carcinomas: somatic inactivation of both alleles in tumors.[J].1999,90(4).
- [13]P L, Broeker,H G, Super,M J, Thirman,etc.Distribution of 11q23 breakpoints within the MLL breakpoint cluster region in de novo acute leukemia and in treatment-related acute myeloid leukemia: correlation with scaffold attachment regions and topoisomerase II consensus binding sites.[J].1996,87.
- [14]Zamponi C,Rustia A,Wierzbicki V,etc.Is exon 5 of the PTEN/MMAC1 gene a prognostic marker in anaplastic glioma?[J].2001,24(2).
- [15]Basso G,Agarwal AM,Real PJ,etc.Mutational loss of PTEN induces resistance to NOTCH1 inhibition in T-cell leukemia.[J].2007,13(10).
- [16]J, Li,C, Yen,D, Liaw,etc.PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer.[J].1997,275.