Effects and expression of KLF4 in leukemic cell line K562
- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1009-9921.2009.03.004
- Key Word:
- 蛋白质类;转录因子;白血病;K562细胞;Proteins;Transcription factors;Leukemia;K562 cells
Abstract： Objective To investigate the expression and effect of zinc-finger protein kuppel-like factor4(KLF4) in leukemic cell line K562. Methods The lever of mRNA and protein of KLF4 is determined with real-time PCR and Western blot in human peripheral monoeyte/macrophage and K562 cells. Results Compared with control group (0.43±0.03), the expression of KLF4 mRNA in K562 cells (0.02±0.01) wassignificant different(P＜0,05). The expression of KLF4 protein in human peripheral monocyte macrophage is higher than that in K562 cells. Conclusion The low expression of KLF4 was present in leukemic cell line K562,which may suggest that KLF4 gene maybe a tumor inhibitor factor, and partially involved in leukemogenesis.
- [1]Mahatan CS,Kraft AS,Geiman DE,etc.The gut-enriched Kruppel-like factor (Kruppel-like factor 4) mediates the transactivating effect of p53 on the p21WAF1/Cip1 promoter.[J].2000,275(24).
- [2]Benjamin D. Rowland,Daniel S. Peeper.KLF4, p21 and context-dependent opposing forces in cancer[J].2006,6(1).
- [3]Cai W,Chen X.Multimodality molecular imaging of tumor angiogenesis.[J].2008,49(Suppl.2).
- [4]Foster KW,Ren S,Louro ID,etc.Oncogene expression cloning by retroviral transduction of adenovirus E1A-immortalized rat kidney RK3E cells: transformation of a host with epithelial features by c-MYC and the zinc finger protein GKLF.[J].1999,10(6).
- [5]Feinberg MW,Cao ZX,Wara AK,etc.Kruppel-like factor 4 is a mediator of proinflammatory signaling in macrophages[J].2005,280(46).
- [6]A, Vidal,A, Koff.Cell-cycle inhibitors: three families united by a common cause.[J].2000,247(1-2).