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Study of DNA polymerase β in chronic myeloid leukemia
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-9921.2008.04.004
- Key Word:
- 白血病,髓样,慢性;DNA聚合酶B;聚合酶链反应;融合蛋白质类,bcr-abl
Abstract： 目的 探讨慢性粒细胞白血病(CML)DNA聚合酶β蛋白表达及其与CML急变机制的关系.方法 用Western blotting方法检测62例CML患者及K562细胞DNA聚合酶β蛋白表达,并与23例健康人作对照;对26例异基因外周血干细胞移植(allo-PBSCT)及12例使用伊马替尼治疗的CML患者用RT-PCR、Western blotting分别动态检测bcr-abl mRNA和DNA聚合酶β蛋白表达;伊马替尼体外作用于CML患者MNC及K562细胞后,用Western blotting方法检测DNA聚合酶B表达及ber-abl融合蛋白磷酸化.结果 与健康人比较,CML患者及K562细胞DNA聚合酶β蛋白的表达明显升高(P＜0.05).allo-PBSCT及伊马替尼治疗后患者DNA聚合酶β蛋白的表达随着bcr-abl mRNA表达的降低而下降.伊马替尼体外作用后,DNA聚合酶β蛋白表达随着bcr-abl融合蛋白酪氨酸磷酸化水平的降低而下降.结论 bcr-abl融合基因增加DNA聚合酶β蛋白表达;DNA聚合酶β蛋白增加可能是CML急变机制之一.
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