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Effect of histone deacetylase inhibitor on B cell lymphoma in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1009-9921.2007.06.005
- Key Word:
- 组蛋白脱乙酰基酶类;淋巴瘤,B细胞;PRDM1;原癌基因蛋白质c-myc
Abstract： 目的 探讨组蛋白去乙酰化酶抑制剂曲古抑菌素A(trichostatin A,TSA)对B细胞淋巴瘤的体外作用机制,为临床应用提供理论依据.方法 选择对多柔比星耐药的B淋巴瘤细胞系SU-DHL-4为研究对象,MTT法检测TSA对其生长抑制作用,RT-PCR检测TSA对转录因子PRDM1和c-myc的影响.结果 纳摩尔浓度的TSA体外作用48h即能够显著抑制SU-DHL-4细胞系的体外增生,且呈剂量依赖性;SU-DHL-4细胞系中表达转录因子PRDM1α和β两种转录本,且比例异常,高表达与肿瘤发生相关的PRDM1β转录以及其下游基因c-myc;150nmol/L TSA作用细胞12h能够显著增加结构功能正常的PRDM1α表达,抑制PRDM1β表达,24h后c-myc表达受抑制.结论 TSA可以显著抑制耐药B淋巴瘤细胞系SU-DHL-4的增生,纠正转录因子PRDM1α和β表达比例,继而抑制下游靶基因c-myc表达是其抗增生作用的机制之一.
- [1]Chadwick RB,Bennington GA,Johnson CK,etc.Candidate tumor suppressor RIZ is frequently involved in colorectal carcinogenesis.[J].2000,97(6).
- [2]Insel RA,Lee SC,Bottaro A.Activation of terminal B cell differentiation by inhibition of histone deacetylation.[J].2003,39(15).
- [3]Takada Y,Gillenwater A,Ichikawa H,etc.Suberoylanilide hydroxamic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing nuclear factor-kappaB activation.[J].2006,281(9).
- [4]Ghosh N,Gyory I,Seto E,etc.Identification of a functionally impaired positive regulatory domain I binding factor 1 transcription repressor in myeloma cell lines.[J].2003,170(6).
- [5]Yi Lin,Kuo-I Lin,Kathryn Calame.Repression of c-myc Is Necessary but Not Sufficient for Terminal Differentiation of B Lymphocytes In Vitro[J].2000,20(23).
- [6]Pasqualucci L,Compagno M,Houldsworth J,etc.Inactivation of the PRDM1/BLIMP1 gene in diffuse large B cell lymphoma.[J].2006,203(2).