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Application of CIK to the patients with refractory or relapsed non-Hodgkin lymphoma
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-9921.2007.04.005
- Key Word:
- 淋巴瘤,非霍奇金;免疫疗法
Abstract： 目的 探讨细胞因子活化杀伤细胞(CIK细胞)用于难治复发性非霍奇金淋巴瘤的安全性及疗效.方法 健康供者及淋巴瘤患者的外周血单个核细胞,经过IFN-γ、CD3单抗和rhIL-2共同作用,体外扩增培养CIK细胞,流式细胞仪检测其免疫表型的改变,MTT法检测CIK细胞的杀伤活性.取培养15～21 d的CIK细胞回输,观察临床疗效及不良反应.结果 健康供者及恶性淋巴瘤患者的外周血单个核细胞可以成功培养出CIK细胞.培养第21天,CIK细胞扩增至52.67～64.81倍.培养期内细胞存活率95%以上.流式细胞仪检测其免疫表型有明显的变化,CD3+ CD56+细胞比例由培养第0天的0.52%～3.04%上升至第21天的48.63%～75.82%,而CD3+ CD8+细胞则由21.2%～33.5%升至82.26%～92.32%.MTT法检测CIK细胞对K562细胞有较高的杀瘤活性,当效靶比为5∶1时,在细胞培养的第21天,CIK对K562细胞的杀伤活力为58.1%～70.9%.在4例接受CIK细胞治疗患者中,治疗前后临床症状有明显的改善,T细胞亚群测定显示CD4/CD8比值(1.03±0.24～1.26±0.31)及CD16+ CD56+细胞(10.91±6.23～15.68±7.42)与治疗前相比明显升高.治疗过程中及治疗后患者无明显不良反应.结论 CIK细胞可以成为难治复发性非霍奇金淋巴瘤的有效治疗手段之一.
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