You Position: Home > Paper
Effect of siRNA on the proliferation in HL-60 cells of leukemic cell line
( views:180, downloads:20 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-9921.2006.04.002
- Key Word:
- c-myc基因;干扰RNA片断;白血病细胞系HL-60细胞增生
Abstract： 目的 研究针对c-myc基因的小分子干扰RNA片断(siRNA)对白血病细胞系HL-60细胞增生的影响,探讨应用siRNA进行白血病基因治疗的可能性.方法 制备特异性对应c-myc基因的siRNA转染HL-60细胞,倒置显微镜观察细胞形态学改变,MTT法检测细胞增生状态,流式细胞仪进行细胞周期分析.结果 siRNA转染HL-60细胞后,细胞生长受抑,增生能力减弱,其增生抑制作用于转染后48 h、72 h最明显,增生抑制率分别为53.2%和51.5%;S期细胞比值由(56.1±4.7)%降至(17.8±3.2)%,细胞阻滞在G0/G1期.结论 针对c-myc基因的siRNA对HL-60细胞增生有明显抑制作用,有望为白血病治疗提供新的途径.
- [1]毕慧,王亚军,何勤.急性白血病细胞C-myc基因表达的研究[J].2003,(2).
- [2]D, Resnitzky,A, Kimchi.Deregulated c-myc expression abrogates the interferon- and interleukin 6-mediated G0/G1 cell cycle arrest but not other inhibitory responses in M1 myeloblastic cells.[J].1991,2(1).
- [3]H, Sumimoto,S, Yamagata,A, Shimizu,etc.Gene therapy for human small-cell lung carcinoma by inactivation of Skp-2 with virally mediated RNA interference.[J].2005,12(1).
- [4]E L, Wickstrom,T A, Bacon,A, Gonzalez,etc.Human promyelocytic leukemia HL-60 cell proliferation and c-myc protein expression are inhibited by an antisense pentadecadeoxynucleotide targeted against c-myc mRNA.[J].1988,85(4).
- [5]S M, Elbashir,J, Harborth,W, Lendeckel,etc.Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.[J].2001,411(6836).
- [6]王松梅,单易非,高红阳,等.反义寡核苷酸对HL60细胞C-MYC基因表达的抑制和诱导分化作用[J].1998,(3).
- [7]Yang D.,Erickson JW.,Lu H..Evidence that processed small dsRNAs may mediate sequence-specific mRNA degradation during RNAi in Drosophila embryos[J].2000,10(19).
- [8]S M, Elbashir,W, Lendeckel,T, Tuschl.RNA interference is mediated by 21- and 22-nucleotide RNAs.[J].2001,15(2).
- [9]Cultraro CM,Borre A,Segal S.c-Myc inactivation by mutant Max alters growth and morphology of NCI-H-630 colon cancer cells.[J].1996,169(1).
- [10]曾嵘.C-MYC的基因调节作用[J].1997,(2).
- [11]P A, Sharp.RNA interference--2001.[J].2001,15(5).