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Construction and identification of mutant HSV1-sr39tk gene eukaryotic expression vector

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Author:
No author available
Journal Title:
JOURNAL OF LEUKEMIA & LYMPHOMA
Issue:
2
DOI:
10.3760/cma.j.issn.1009-9921.2006.02.001
Key Word:
突变型自杀基因HSV1-sr39tk;真核表达载体

Abstract: 目的克隆突变型单纯疱疹病毒胸苷激酶基因HSV1-sr39tk并构建其真核表达载体.方法通过PCR扩增HSV1-sr39tk基因,用BamH Ⅰ和BglⅡ双酶切纯化的PCR产物,在T4DNA连接酶作用下于室温将其连接于经BamH Ⅰ单酶切的慢病毒载体转移质粒pTK151上,重组质粒经Pst Ⅰ酶切及测序鉴定.结果阳性重组质粒酶切及测序鉴定与预期结果完全相符.结论成功克隆出突变型自杀基因HSV1-sr39tk,并成功构建其真核表达载体.

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