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Study on ultrasound induced apoptosis of leukemia cell K562 cultured in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1009-9921.2006.01.003
- Key Word:
- 超声体外诱导;白血病细胞K562凋亡
Abstract： 目的研究超声对诱导白血病细胞K562凋亡的作用,为超声治疗的临床应用提供实验依据.方法以人慢性粒细胞白血病急变细胞株K562为研究对象,用频率为1.8 MHz的超声波辐照白血病细胞K562,用光学显微镜观察凋亡细胞形态学改变,流式细胞仪检测细胞凋亡率.结果超声辐照K562细胞后24 h,8 mV × 5 min组及8 mV × 10 min组细胞凋亡率分别为2.3%和8.28%;10 mV×5 min及10 mV × 10 min组细胞凋亡率分别为9.36%和21.46%.电压为7mV时,超声辐照K562细胞后0.5 h,1 h,3 h,5 h细胞凋亡率分别为1.9%,3.6%,5.7%,7.7%.同时光学显微镜下可见凋亡小体形成等典型形态学改变.结论超声可以诱导白血病K562细胞凋亡.
- [1]Ng Kayun,Liu Yang.Therapeutic ultrasound: its application in drug delivery.[J].2002,22(2).
- [2]ter-Haar GR.High intensity focused ultrasound for the treatment of tumors.[J].2001,18(4).
- [3]Flavio Lejbkowicz,Samuel Salzberg.Distinct Sensitivity of Normal and Malignant Cells to Ultrasound in Vitro[J].1997,105(s6).
- [4]McDannold N,Bronson RT,Vykhodtseva N,etc.Apoptosis in ultrasound-produced threshold lesions in the rabbit brain.[J].2001,27(1).
- [5]Olivo M.Bio-distribution and subcellular localization of Hypericin and its role in PDT induced apoptosis in cancer cells.[J].2002,21(3).
- [6]Gutierrez PL.,Harrison GH.,Balcerkubiczek EK..In Vitro Action of Continuous-Wave Ultrasound Combined with Adriamycin, X Rays or Hyperthermia[J].1996,145(1).
- [7]V, Misík,P, Riesz.Free radical intermediates in sonodynamic therapy.[J].2000,899.