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Intracrine role of EDAG in leukemia cell line K562
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-9921.2005.04.002
- Key Word:
- 自律性生长;细胞内自激因子;核蛋白;EDAG;白血病细胞系
Abstract： 目的为肿瘤细胞自律性生长的细胞内自激因子假设提供实验证据.方法将新发现的造血相关核蛋白EDAG基因转染人白血病细胞系K562,比较过表达EDAG对K562细胞的增生能力,以及对细胞的造血调控、凋亡及细胞周期相关蛋白表达的影响.结果在无血清培养条件下,过表达EDAG的K562细胞的增生能力明显高于两组对照细胞,并且有c-myb和bcl-2 mRNA表达的显著上调.结论过表达的EDAG可以起细胞内自激因子作用.为核蛋白异常表达可以起细胞内自激因子作用提供了实验证据.
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