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Studying of effects of meisoindigo on acute promyelocytic leukemia cell line NB4 cells in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1009-9921.2005.03.003
- Key Word:
- 甲异靛;NB4细胞系;增生;凋亡;分化
Abstract： 目的探讨甲异靛对NB4细胞系的抑制增生、诱导凋亡和分化的作用.方法用锥虫蓝染色方法进行活细胞计数;用NBT还原实验和CD11a、CD11b的表达判定促分化作用;光镜和电镜细胞形态学、TUNEL标记和Sub-G1测定观察细胞凋亡,RT-PCR和流式细胞术分析法检测凋亡调控基因mRNA及蛋白水平表达.结果20μmol/L和30μmol/L甲异靛可明显抑制NB4细胞的增生,诱导细胞凋亡并下调bcl-2表达,使细胞停滞在G0/G1期.5μmol/L和10μmo1/L时NB4细胞的NBT还原能力明显升高,CD11a表达明显升高.结论甲异靛通过诱导细胞凋亡和使细胞停滞在G0/G1期这两种机制显著抑制NB4细胞增生,此外,还有部分诱导细胞分化的作用.
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