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ESTABLISHMENT AND CHARACTERISTICS OF MURINE MULTI- DRUG RESISTANT LEUKEMIA CELL LINE
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1009-9921.2004.02.004
- Key Word:
- 白血病细胞;小鼠;多药耐药;长春新碱
Abstract： 目的:建立小鼠多药耐药白血病细胞系P388/VCR并研究其生物学特性.方法:小鼠淋巴细胞白血病细胞系P388,通过长期、单一、逐步提高浓度的方法建立多药耐药的白血病细胞系P388/VCR.采用悬浮细胞培养和半固体培养观察该细胞系的生长规律;流式细胞术分析细胞周期分布及柔红霉素在细胞内的积聚量;RT-PCR方法检测mdrla基因表达;四氮甲基唑蓝(MTT)法研究P388/VCR细胞系的耐药谱.结果:经过6个月单一、小剂量及间歇给药的方法诱导P388细胞成为多药耐药细胞系P388/VCR.与亲本细胞系P388比较,细胞生长曲线及倍增时间无明显差异,软琼脂培养集落形成率及大集落形成率差异不显著,细胞周期分析P388/VCR细胞S期比例降低.P388/VCR细胞除对原诱导药物具有耐药性外,对其他多种抗肿瘤药物如柔红霉素、高三尖杉酯碱、米托蒽醌、足叶乙苷等也具有交叉耐药性.流式细胞仪检测细胞内柔红霉素含量P388/VCR细胞平均荧光值为3.72,明显低于P388细胞的1731.RT-PCR检测mdrla基因的表达结果为:P388细胞阴性,P388/VCR细胞阳性.结论:多药耐药细胞系P388/VCR具有mdrla基因表达阳性及对多种抗肿瘤药物耐药的特征.
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