You Position: Home > Paper

STUDY OF TELOMERASE ACTIVITY AND MICROSATELLITE STABILITY DURING IN VITRO EXPANSION OF CORD BLOOD CD+34 CELLS

( views:158, downloads:8 )
Author:
No author available
Journal Title:
JOURNAL OF LEUKEMIA & LYMPHOMA
Issue:
5
DOI:
10.3760/cma.j.issn.1009-9921.2003.05.001
Key Word:
脐血CD+34细胞;端粒酶;微卫星

Abstract: 目的:通过检测脐血CD+34细胞扩增前后端粒酶活性及微卫星DNA序列,探讨其遗传稳定性.方法:用Mini MACS磁分离技术分离脐血CD+34细胞,体外扩增2周,PCR-ELISA方法检测扩增前后细胞的端粒酶活性;用PCR银染方法对脐血CD+34细胞扩增前后5个微卫星序列进行比较分析.结果:脐血CD+34细胞体外扩增过程中,端粒酶活性呈现先升后降的趋势,0天时,端粒酶活性相对值的中位数为14%,第7天明显增高,达57%,第14天回落至29%;扩增前后,BAT-25、Mfd41、D5S436、D5S396、D2S123 5个微卫星位点未见改变.结论:脐血CD+34细胞扩增过程中,端粒酶活性会暂时性升高,所测的微卫星位点未见改变.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn