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EXPERIMENTAL STUDY ON PURGING ACUTE MYELOGENORS LEUKEMIA MARROW IN VITRO WITH THE COMBINATION OF rhTNF-α AND IL-2
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- Author:
- No author available
- Journal Title:
- JOURNAL OF LEUKEMIA & LYMPHOMA
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1009-9921.2001.02.005
- Key Word:
- 白血病;髓细胞性;急性;肿瘤坏死因子α;白细胞介素2;骨髓净化
Abstract： 目的:探讨重组人肿瘤坏死因子α(rhTNF-α)联合白细胞介素2(IL-2)体外净化AML骨髓方案的可行性及其作用机制.方法联合rhTNF-α(1×106U/L)和IL-2(5×105U/L),对17例AML患者骨髓体外净培养1天～3天,采用半固体培养法测定粒-巨噬细胞集落(CFU-GM)、白血病祖细胞集落(CFU-L)形成,MTT细胞毒性测定及免疫组化法测定bcl-2阳性率的变化.结果①rhTNF-α与IL-2联合后,对CFU-L有选择性抑制作用,而对CFU-GM无明显影响;②rhTNF-α和IL-2对白血病细胞有协同杀伤作用,净化培养3天后,bcl-2阳性率明显降低.结论联合rhTNF-α和IL-2净化效果好;介导细胞毒性,下调bcl-2表达,诱导白血病细胞凋亡,可能是其净化骨髓的机制之-.
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