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The study of killing and proapoptotic effects of topoisomerase I inhibitor on L1210/CDDP cells

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Author:
No author available
Journal Title:
CHINA CLINICAL PRACTICAL MEDICINE
Issue:
3
DOI:
10.3760/cma.j.issn.1673-8799.2009.03.003
Key Word:
拓扑替康;L1210/CDDP细胞株;凋亡;Caspase-8;Caspase-3;Topotecan;L1210/CDDP cell line;Apoptosis;Caspase-8;Caspase-3

Abstract´╝Ü Objective Observation and Study of Killing and Proapoptotic Effects of Topoisomerase Ⅰ (topotecan) inhibitor on L1210/CDDP cells. Methods Analyze Topotecan' s killing activity and inductive effect on L1210/CDDP cell lines and the relationship between the above two and Caspase, using MTF method,Annexin V FITC staining, DNA ladder electrophoresis and cell morphology methods. Results TPT on L1210/CDDP cell line has strong anti-role, and a clear time-dependence. At the same time, the target cells present a sign of apoptosis. Caspase-specific inhibitor can cause a certain effect on apoptosis. Conclusion Topotecan' s killing activity and inductive effect on L1210/CDDP cell lines can result in activation of Caspase-8 and Caspase-3.

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