Abstract： Objective To observe the imaging features of short-wave length fundus autofluorescence (SW-AF),near-infrared fundus autofluorescence (NIR-AF)and spectral-domain optical coherence tomograph (SD-OCT) in acute central serous chorioretinopathy (CSC).Methods This is a retrospective observational consecutive case series study.Eighty-two eyes with acute CSC from 76 patients were enrolled in this study.There were 50 males (56 eyes) and 26 females (26 eyes),average age was (41.32 ± 7.37) years old,average course of the disease was (0.47 ± 1.73) months.The routine clinical examinations included best corrected visual acuity,slit lamp microscope,indirect ophthalmoscope,fundus fluorescein angiography (FFA),SW-AF,NIR AF and SD-OCT.The imaging features of NIR-AF,SW-AF,SD-OCT and FFA in same eye with acute CSC were analyzed comparatively.Results SD-OCT showed retinal pigment epithelium (RPE) detachment corresponding to RPE leakage point on FFA images in all CSC eyes.RPE leakage points in all eyes corresponding to local hypo-NIR-AF,whereas corresponding to normal SW-AF.NIR-AF demonstrated the area of decreased AF corresponding to serous retinal detachment in all eyes,of which,the area of hypo-SW-AF was consistent with that of sensory retinal detachment in 75 eyes.Except for RPE leaking fluorescein in 82 eyes,FFA showed window defects corresponding to clusters of hypo-NIR-AF in 45 eyes,of which,27 eyes showed hypo-SW-AF appearing concurrently with the locations of window defects of FFA.In 18 eyes,the extent and amount of hypo-SW-AF were less than that of hypo-NIR-AF.Conclusions There was local RPE detachment in all eyes with acute CSC.The locations of sensory retinal detachment,local RPE detachment and RPE depigmentation showed hypo-NIR-AF.The locations of sensory retinal detachment and RPE depigmentation showed hypo-SW-AF in most of acute CSC eyes.