Abstract： Objective To observe the functional and morphological changes of macular after panretinal photocoagulation(PRP)in the patients with diabetic retinopathy(DR). Methods A total of 57 eyes of 34 patients with DR undergoing PRP were enrolled in this prospective and self-reflection study. Comparatively analyze the changes of the best visual acuity(BCVA), optical coherence tomography (OCT) and multi-focal electroretinography (mfERG) before PRP, 20 days, 3 months and more than 9 months after PRP. Statistical analyses were performed by wilcoxon, chi-square, Dunnett-t, LSD-t tests and spearman related analyses. The changes of macular function and foveal retinal thickness before and after PRP were comparatively analyzed. Results BCVA of all patients reduced at 9 months after PRP(P= 0. 022). The amplitude density of mfERG P1 of ring 2 decreased at 20 days after PRP(P= 0. 039), then recovered at 3 months and decreased again at 9 months(P=0. 014). The amplitude density of mfERG P1 of ring 3-5 decreased at 20 days, 3 months and more than 9 months after PRP(20 days: ring 3: P=0. 000, ring 4: P= 0. 001, ring 5:P=0.000; 3 months: ring 3:P=0. 000, ring 4. P=0. 006, ring 5: P=0.001; more than 9 months: ring 3: P=0. 000,ring 4: P=0.000, ring 5: P=0. 000). The amplitude density of mfERG P1 of ring 1 was significantly lower at 9 months after PRP(P=0. 050). The foveal retinal thickness increased at 20 days after PRP(P = 0. 007), then recovered at 3 months or later. Cystoid macular degeneration was found in 6 eyes(10. 5%) at 20 days after PRP. Conclusions After the treatment of PRP, there were some extend reduction of the macular function, a transient increase on foveal retinal thickness. Combined mfERG and OCT can be a comprehensively and objectively assessment of macular function and morphology.