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Experimental study on photodynamic induced anterior ischemic optic neuropathy in rat animals

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
Issue:
2
DOI:
No doi available
Key Word:
视神经病变,缺血性/病理学;光化学疗法;模型,动物;Optic neuropathy,ischemic/pathology;Photochemotherapy;Models,Animal

Abstract: Objective To establish an rat model of the Anterior Ischemic Optic Neuropathy (rAION),and identify its reliability by observing the fundus,fluorescein fundus angiography(FFA),optical coherence tomography(OCT),visually evoked potential(VEP)and histopathology. MethodsThirty male Sprague-Dawley rats were randomly divided into group Naive with 5 rats,group Laser with 5rats,group hematoporphyrin derivative(HPD)with 5 rats,group rAION with 15 rats.All of the right eyes were the experimental eyes and the left ones were the control.after administration of HPD in rats'vena caudalis.The rats in group Laser were treated with 8 krypton red 647nm/2/3disc spot laser for 120seconds,the rats in group HPD were treated by administration of HPD in rats'vena caudalis,and the rats in group Na?ve were not treated. Results From 1 day to 6 days after rAION induction,the ON was pale and swollen in the superior part.The ON at 90 days after induction was pale and shrunken.30minutes after rAION induction,hvperfluorescence appeared in the superior part of the optic disc,and the hypofluorescence in the 23rd day.In early FFA,hypofluorescence appeared at the isehemic area of the optic disc,and in midst and later stage the isehemie area revealed hvperfluorescence in the 1st day after rAION induction,the hvpofluorescence in midst and later stage in the sixth day after r-AION model.The latent period of F-VEP expanded.The amplitude cut down in the 1-2 days after r-AION induction and did not changed in 35nd day.The surface of optic disc showed higher and rougher than the surface of retina in sections,in high power field the optic disc showed edema with the displacement of retina surrounding the disc 1 day after treatment.Rarefaction and degeneration in the nerve fiber of retina and reduction of the number of nuclei of ganglion cells in the 23st day after the modelinduction,and the thinning of nerve fiber of the optic disc and its surroundings.In contrast,there was no change in group Na?ve,group Laser and group HPD. Conclusions The r-AION model is like the human AION in fundus,FFA,OCT,VEP and histopathology.The rAION model provides the ischemic changes of occurrence of AION,and is helpful for the fundamental study of the AION.

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