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Overexpression of Bax and induction of apoptosis in human retinal pigment epithelial cell lines
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 2
- DOI:
- 10.3760/j.issn:1005-1015.2001.02.016
- Key Word:
- 色素上皮,眼;细胞死亡;基因;流式细胞术
Abstract： 目的研究外源性促凋亡基因bax过表达对培养人的视网膜色素上皮(retinal pigment epithelium ,RPE)细胞凋亡的影响.方法通过脂质体Lipofectin介导法用含入全长bax基因片段的真核表达载体PMDNA3-hbax质粒转化体外培养的RPE细胞,用流式细胞仪检测细胞周期的变化,提取实验组细胞DNA进行DNA梯度电泳. 结果 Zn2+可诱导PMDNA3-hbax转化组RPE细胞出现明显的凋亡峰,凋亡峰位于单倍体和二倍体之间,凋亡率为36%,DNA电泳可见DNA梯状条带,PMDNA3空载体组及未转染组RPE细胞未见亚二倍峰和DNA梯状条带出现.结论外源性促凋亡基因bax过表达可诱导RPE细胞凋亡.
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