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Differentiation of embryonic stem cells into neurons and retina-like structure in nude mice
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1673-4386.2000.03.003
- Key Word:
- 干细胞;胚胎;神经元;视网膜;细胞移植
Abstract： 目的 研究小鼠胚胎干细胞在裸鼠眼内的生长特性.方法 将未分化的胚胎干细胞移植到Balb/c裸小鼠眼内.14～20 d处死裸鼠,观察其形态学和免疫组织化学变化.结果 胚胎干细胞移植到裸鼠眼内,在眼前房和玻璃体腔内能见到黄白色物,逐渐增大.光镜检查:在前房和玻璃体腔内均能见到未分化细胞和已分化的细胞,部分细胞的形态和排列方式与视网膜组织中的一些结构相似,其免疫组织化学抗神经元特异性烯醇化酶染色大部分已分化细胞呈强阳性反应.结论 胚胎干细胞在裸小鼠眼内的特定环境中,向神经元和视网膜样结构分化.
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