You Position: Home > Paper

Fibroblast growth factor 10 stabilizes microtubule and maintains neuronal survival through Rho A/ROCK signal pathway

( views:0, downloads:0 )
Author:
No author available
Journal Title:
Chinese Journal of Neuromedicine
Issue:
2
DOI:
10.3760/cma.j.cn115354-20220629-00453
Key Word:
成纤维细胞生长因子10;微管;髓鞘碎片;RAS同源基因家族成员A;Rho A相关蛋白激酶;Fibroblast growth factor 10;Microtubule;Myelin debris;RAS homologous gene family member A;Rho A-associated protein kinase

Abstract´╝Ü Objective:To investigate the protective effect of fibroblast growth factor 10 (FGF10) on neuronal injury and its potential molecular mechanism.Methods:Cortical neurons were dissected from brain tissues of newborn SD rats and seeded on Poly-L-Lysine coated plates. These neurons were then divided into control group, myelin group and myelin+FGF10 group; after 4 h of culture, neurons in the myelin group were added with a certain content of myelin solution (final concentration: 10 μg/mL), while neurons in the myelin+FGF10 group were added with myelin and FGF10 solution (final concentration: 4.3 nmol/L). One week after culturing, the neuronal apoptosis was detected by TUNEL and flow cytometry; neuronal survival was evaluated by live/dead assay and CCK-8 assay; expressions of apoptosis-related proteins, microtubule related proteins and RAS homologous gene family member A (Rho A)/Rho a-related protein kinase (ROCK) signaling pathway related proteins were detected by Western blotting and immunofluorescent double-label staining.Results:Compared with the control group, the myelin group had significantly increased neuronal apoptosis rate by TUNEL, early neuronal apoptosis rate by flow cytometry, activated cysteine proteinase-3 (caspase-3) protein expression and neuronal mortality rate by live/dead assay, and significantly decreased Bcl-2/Bax value, neuronal survival rate by CCK-8 method, value of acetylated tubulin/Tyr-tubulin (Ace/Tyr-tubulin), Tau protein expression and Ace/Tyr-tubulin fluorescent intensity ratio, and statistically increased Rho A and ROCK protein expressions and Rho A fluorescent intensity ( P<0.05). Compared with the myelin group, the myelin+FGF10 group had significantly decreased neuronal apoptosis rate by TUNEL, early neuronal apoptosis rate by flow cytometry and activated caspase-3 protein expression, significantly increased Bcl-2/Bax value, neuronal survival rate by CCK-8 method, Ace/Tyr-tubulin value, Tau protein expression and Ace/Tyr-tubulin fluorescent intensity ratio, and statistically decreased Rho A and ROCK protein expressions and Rho A fluorescent intensity ( P<0.05). Conclusion:FGF10 maintains microtubule stability in neurons likely through inhibiting Rho A/ROCK axis to antagonize myelin-induced apoptosis and improve the neuronal survival.

  • This article has no references!
WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn