Abstract： Objective:To understand the current situation of long working hours exposure of couriers in Zhejiang Province , and to analyze the influence of long working hours exposure levels on their occupational stress and depression symptoms , and provide a basis for promoting the physical and mental health of couriers. Methods:From September to December 2021 , 1159 couriers from Zhejiang Express Transport Companies were selected as respondents by cluster sampling , and their basic information were collected. The occupational stress and depression symptoms of the couriers were assessed using the Core Occupational Stress Scale (COSS ) and the Patient Health Questionnaire-9 (PHQ-9 ). The effects of long work hours (>48 h per week ) on the occupational stress and depression symptoms of the couriers were analyzed. Results:The average age of 1159 courier in Zhejiang Province was (33.24±8.42 ) years , the average weekly working hours were (63.21±18.77 ) h , and 75.15% (871/1159) were long-term workers. The detection rates of occupational stress and depression symptoms in courier were 32.44% (376/1159) and 32.53% (377/1159), respectively. There were significant differences in the detection rates of occupational stress and depression symptoms among couriers with different ages, educational background, marital status, monthly average income, length of service, position or post, shift status, drinking status, and average weekly working hours ( P <0.05). The adjusted logistic regression analysis showed that, after adjusting for the influence of confounding factors such as age, educational background, marital status, monthly average income, length of service, position or post, shift status and drinking status, compared with the weekly working hours ≤48 h, weekly working hours of 63-77 h, 78-92 h, ≥93 h were the risk factors for occupational stress ( OR=1.547, 95% CI: 1.019-2.350; OR=1.886, 95% CI: 1.184-3.006; OR=2.338, 95% CI: 1.188-4.062) and depression symptoms ( OR=1.897, 95% CI: 1.258-2.860; OR=2.041, 95% CI: 1.290-3.230; OR=4.978, 95% CI: 2.551-9.715) of couriers ( P<0.05) . Conclusion:Long working hours could increase the risk of occupational stress and depression symptoms among couriers. It is necessary to arrange working hours reasonably to reduce the occurrence of occupational stress and depression symptoms of couriers.