Abstract： Malaria is an endemic infectious disease caused by plasmodium and is mainly prevalent in tropical and subtropical areas. The World Health Organization certificated malaria elimination in China in 2021, however, the threat of imported malaria will be persisting as international exchanges become more frequent. In order to promote further understanding and rational diagnosis and treatment of malaria for clinicians, Chinese experts in the field of infectious diseases and parasitic diseases were invited to jointly develop this guidelines. The guidelines summarize the etiology, epidemiology, pathogenesis, clinical manifestations, diagnosis and differential diagnosis, treatment, nursing, and prevention of malaria, and therapeutic options for different clinical conditions are also introduced, so that the clinician can use it properly.