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Advances in carbapenemase detection in Gram-negtive bacilli

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Author:: No author available

Journal Title:: Chinese Journal of Clinical Infectious Diseases

Issue:: 3

DOI:: 10.3760/cma.j.issn.1674-2397.2016.03.008

Key Word:: 革兰氏阴性菌;碳青霉烯酶;耐药性;表型;基因型;Gram-negative bacteria;Carbapenemase;Drug resistance;Phenotype;Genotype

Abstract： With increasing use of carbapenem antibiotics , carbapenems-resistant gram-negative bacteria are spreading, and carbapenemase-producing is the main mechanism of carbapenems resistance . Rapid and accurate identification of carbapenemase and its type is of great importance to timely and effective treatment and control of infections .Chromogenic /Fluorogenic culture media, modified Hodge test and double disk synergy test are traditional methods for carbapenemase detection , but all are time-consuming. Biochemical method is more time efficient and with high sensitivity and specificity , but cannot be used to identify subtypes.Now matrix-assisted laser desorption ionization -time-of-flight mass spectrometry (MALDI-TOF MS) has been successfully applied in the identification of species , subtypes and detection of drug -resistant genes.And among various carbapenemase gene detection techniques , next generation sequencing (NGS) can also be used for the detection of integrons , transposons and plasmids, which is important in both epidemiology and resistant mechanism studies .This article reviews the advantages and disadvantages of various methods for phenotype and gene detection of carbapenemase .

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