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Comparative evaluation of disk diffusion and E-test methods for clarithromycin susceptibility testing of Helicobacter pylori

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Author:: No author available

Journal Title:: Chinese Journal of Clinical Infectious Diseases

Issue:: 3

DOI:: 10.3760/cma.j.issn.1674-2397.2014.03.006

Key Word:: 螺杆菌,幽门;克拉霉素;纸片扩散抗菌试验;E-test;Helicobacter pylori;Clarithromycin;Disk diffusion antimicrobial tests;E-test

Abstract： Objective To compare disk diffusion with E-test methods for clarithromycin susceptibility testing of Helicobacter pylori (H.pylori).Methods A total of 44 strains of H.pylori were isolated from gastric mucosa biopsy from patients undergoing gastroscopic examination.Disk diffusion and E-test methods were used for clarithromycin susceptibility testing of H.pylori.The agreement of disk diffusion and E-test was assessed by linear regression analysis.Results The minimum inhibitory concentration (MIC) tested by E-test method ranged from 0.016 to 256 μg/mL,and drug resistance was observed in 12(27.3％) isolates.In range of 0-35 mm of inhibition diameter,the results of disk diffusion method were correlated well with the MICs obtained by E-test method (r2 =0.91,P ＜0.01).Regression analysis showed that with inhibition diameters≥ 18 mm as considered sensitive to clarithromycin and ≤ 15 mm as resistant,the agreement was 100％ between two methods.Conclusion The disk diffusion method is equivalent to the E-test method for clarithromycin susceptibility testing of H.pylori,which can be an alternative method for clinical application.

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