Characterization of resistance to β-lactams in clinical isolates of Klebsiella pneumoniae
- Author:
- No author available
- Journal Title:
- Chinese Journal of Clinical Infectious Diseases
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1674-2397.2011.05.008
- Key Word:
- 克雷伯菌,肺炎;β-内酰胺酶基因;可移动遗传元件;指标聚类分析;Klebsiella pneumoniae;β-lactamase genes;Mobile genetic dements;Index cluster analysis
Abstract： Objective To investigate correlation between drug-resistance related genes and mobile genetic elements of Klebsiella pneumoniae resistant to β-lactams. Methods Forty-seven strains of multidrug-resistant Klebsiella pneumoniae were collected from 6 hospitals in Hangzhou and Huzhou of Zhejiang province from August 2008 to May 2010.Modified Hodge test was performed to detect phenotypes of carbapenemases.Forty kinds of β-lactamases (class A-D),ompK35,ompK36,and 12 kinds of mobile genetic elements were detected by PCR,and the results were analyzed by index cluster.Results Thirty-five strains were positive in modified Hodge test,and 5 kinds of β-lactamases gene ( including KPC-2-like,GenBank:HQ258934) and 9 kinds of mobile genetic elements were detected.Mutations were observed in ompK35 and ompK36 when compared with sensitive strains.Index cluster analysis showed that correlation existed between KPC-2,KPC-2-like and ISKpn6,between TEM-1 and ISEcpl,IS26,int Ⅰ 1,trbC,IS903,and between CMY-2,OXA-30,DHA-1 and tnpU,tnp513,trbC.Conclusions Five kinds of β-1actamases genes,and mutations in ompK35 and ompK36 may be associated with the resistance to β-1actams in multidrug-resistant Klebsiella pneumoniae.
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