You Position: Home > Paper

Effect of rat endothelial progenitor cell on proliferation and apoptosis of bone marrow mesenchymal stem cell

( views:0, downloads:0 )
Author:
No author available
Journal Title:
Chinese Journal of Stomatology
Issue:
2
DOI:
10.3760/cma.j.issn.1002-0098.2014.02.009
Key Word:
内皮细胞;间质干细胞;细胞增殖;细胞凋亡;共同培养技术;Endothelial cells;Mesenchymal stem cells;Cell proliferation;Apoptosis;Coculture technique

Abstract´╝Ü Objective To investigate the effect of endothelial progenitor cell (EPC) on regulating proliferation and apoptosis of bone marrow mesenchymal stem cell (BMSC) in the indirect co-culture system.Methods BMSC and EPC were cultivated and identified in vitro.Using transwell inserts to establish EPC and BMSC indirect co-culture system.Experimental groups were prepared as follows:BMSC/EPC indirect co-culture:BMSC were co-cultured with EPC that were separated in transwell insert.Control:BMSC/BMSC were indirect co-cultured at the same cell counts with the experimental groups.Colony forming unit-fibroblast(CFU-F) assay was studied with three types of EPC/BMSC ratios (1∶1,10∶ 1,100∶1) to assess the capacity and efficiency for cell self-renewal.In addition,flow cytometry technique was used to detect the cell cycle at co-cultured 3 days and the cell apoptosis at co-cultured 3,7,10 d.Results Under indirect co-culture condition,EPC could significantly promote cell cycle progress and enhance capacity of cell self-renewal.Co-cultured EPC resulted in an accumulation of BMSC at S phase [experimental group:(15.72 ±2.93)%,control group:(2.02 ±0.66)%,P <0.01].CFU-F assay showed that the self-renewal capacity of EPC:BMSC/10:1 group [(50.98 ± 6.32) %] and 100 ∶ 1 group [(57.87 ± 14.06) %] were significantly higher than that in control group [(33.07 ± 9.60) % and (30.06 ± 7.20) %] (P < 0.01).However,EPC had a slight but non-significant down-regulated effect on BMSC apoptosis (P > 0.05).Conclusions Under indirect co-culture condition,EPC could enhance the proliferation of BMSC,but could not regulate cell apoptosis in vitro.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn