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The comparison of the ability of polysaccharide from different Porphyromonas gingivalis in inducing THP-1 cells to produce cytokines

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Author:
No author available
Journal Title:
Chinese Journal of Stomatology
Issue:
2
DOI:
10.3760/cma.j.issn.1002-0098.2014.02.004
Key Word:
紫单胞菌,龈;多糖;细胞因子类;THP-1细胞;Porphyromonas gingivalis;Polysaccharides;Cytokines;THP-1 cells

Abstract´╝Ü Objective To compare the ability of the polysaccharide from various Porphyromonas gingivalis(Pg) type and clinical strains in inducing THP-1 cells to produce cytokines interleukin (IL)-1 β,IL-8,and tumor necrosis factor (TNF)-or,in order to analyze the immunogenicity of Pg polysaccharide components and the virulence-associated factors of this periodontal pathogen.Methods The bacterial polysaccharide was extracted from high virulent Pg strains,W83,SJD2,SJD12 and low virulent Pg,ATCC33277,SJD4,SJD5,and SJD11 by phenol-water extraction.The extracted polysaccharide was used to stimulate the THP-1 cells with different simulation periods and doses.The level of the cytokines,including IL-1 β,IL-8 and TNF-α in the cell culture suspension was measured by enzyme-linked immunosorbent assay (ELISA).Results The polysaccharide extraction of Pg strains was composed of lipopolysaccharide (LPS) and capsular polysaccharide.The secretion of IL-1 β,IL-8 and TNF-α,produced by the THP-1 cells showed in a time-and dose-dependent manner in the medium containing 10% fetal bovine serum.The level of these cytokines of the high virulent strains was higher than that of the low virulent strains in medium containing 1% fetal bovine serum.Four hours after stimulation with polysaccharide extracted from high virulent strains,the levels of IL-1β,IL-8,and TNF-α in the cell suspension were (1 639 ± 497),(1 648 ± 513) and (140 ±48) μg/L,respectively,whereas for low virulent strains,the levels of IL-1β,IL-8,and TNF-o were (773 ±382),(892 ± 400) and (67 ± 33) μg/L,respectively.Conclusions Polysaccharide extracted from Pg could induced the THP-1 cells to secrete the cytokines of IL-1β,IL-8 and TNF-α.The level of the cytokines produced by the THP-1 cells associates with the bacterial virulent properties.

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