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Human β-defensin-3 regulates the proliferation and the secretion of prostaglandin E2 and matrix metalloproteinase-1 in human gingival fibroblasts

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Author:: No author available

Journal Title:: Chinese Journal of Stomatology

Issue:: 12

DOI:: 10.3760/cma.j.issn.1002-0098.2013.12.008

Key Word:: β防御素;前列腺素E类;基质金属蛋白酶1;牙龈成纤维细胞;Beta-defensins;Prostaglandins E;Matrix metalloproteinase 1;Gingival fibroblasts

Abstract： Objective To observe the effect of human β-defensin-3 (HBD-3) on proliferation and the secretion of prostaglandin E2(PGE2) and matrix metalloproteinase-1 (MMP-1) in human gingival fibroblasts(HGF).Methods The HGF were cultured with tissue-explant method and the fourth-generation HGF were plated in 96-well plate.All groups except the control group were treated with different concentrations of HBD-3 for 7 days.Then the HGF proliferation was evaluated with methyl thiazolyl tetrazolium(MTT) colorimetry and the secretions of PGE2 and MMP-1 at the 12th hours of each group were detected by enzyme-linked immunosorbent assay(ELISA).Results The result of MTT dynamic monitoring showed that the amount of HGF increased with time in all groups in concentration dependent manner.ELISA showed that the secretions of PGE2 and MMP-1 in 1.0 mg/L HBD-3 group were (350.56 ± 63.96) ng/L and (13.22 ±0.59) μg/L,significantly higher than those in the control group and 10.0 mg/L HBD-3 group (P ＜ 0.05).Conclusions HBD-3 promoted the proliferation of HGF.The low concentration of HBD-3 may play a role in immunoregulation through increasing the secretions of PGE2 and MMP-1.

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