Effect of Porphyromonas gingivalis lipopolysacchearide on the expression of CC chemokine receptor 2 in monocytes
- Author:
- No author available
- Journal Title:
- Chinese Journal of Stomatology
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.1002-0098.2013.07.004
- Key Word:
- 动脉粥样硬化;受体,趋化因子;紫单胞菌,龈;脂多糖类;Atherosclerosis;Receptors,chemokine;Porphyromonas gingivalis;Lipopolysaccharides
Abstract： Objective To investigate the effects of Porphyromonas gingivalis lipopolysacchearide (Pg-LPS) on the expression of CC chemokine receptor 2 (CCR2) in THP-1 monocyte and to explore the relationship between periodontitis and cardiovascular disease in molecular level.Methods THP-1 monocytes were incubated with different concentrations of Pg-LPS(10,100,1000 μg/L) for 1,4 and 24 h respectively,then flow cytometry and reverse transcription-PCR were adopted to determine cell surface protein levels and mRNA levels of CCR2.Results The protein levels and mRNA levels of CCR2 were higher in all experiment groups of 1 h and 4 h than that in the control group(P ＜ 0.05),except the protein expression of CCR2 in T1 group of 1 h (55.74 ± 0.96).The protein expression (52.56 ± 0.61,40.98 ± 0.86,26.50 ± 0.67) and mRNA levels(0.095 ±0.006,0.070 ±0.004,0.046 ±0.004) of CCR2 were lower in all experiment groups than that in the control group (56.99 ± 0.44,0.104 ± 0.003) at 24 h (P ＜ 0.05).The protein levels and mRNA levels of CCR2 were increased in all experiment groups at 4 h and reduced at 24 h (P ＜ 0.05).Conclusions Pg-LPS can upregulate CCR2 expression on THP-1 monocyte surface in concentration dependent manner in early stage,promoting the monocyte chemoattractant.Periodontitis may promote atherosclerosis by enhancing monocyte chemoattractant through periodontal pathogens.
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