17-β estradiol promotes the expression of interleukin-6 in human periodontal ligament cells infected with Porphyromonas gingivalis
- Author:
- No author available
- Journal Title:
- Chinese Journal of Stomatology
- Issue:
- 6
- DOI:
- 10.3760/cma.j.issn.1002-0098.2012.06.003
- Key Word:
- 雌二醇;紫单胞菌,龈;人牙周膜细胞;炎症因子;Estradiol;Porphyromonas gingivalis;Human periodontal ligament cells;Inflammatory factors
Abstract： Objective To investigate the effects of 17-β estradiol (E2) and Porphyromonas gingivalis(Pg)W83 on the expression of interleukin( IL)-6 and IL-8 in human periodontal ligament cells (hPDLC).Methods Primary cultures of hPDLC were established and the cells of passage four were treated with 10-10 mol/L E2,10 -7 mol/L E2 or PgW83 individually or E2 combined with PgW83.The expression levels of IL-6 and IL-8 protein at 12 h and 24 h were measured with enzyme-linked immunosorbent assay and the levels of mRNA at 24 h were detected with real-time reverse transcriptase polymerase chain reaction.Results The expression level of IL-6 reached (2482.88 ± 26.53 ) ng/L in hPDLC treated with Pg at multiplicity of infection(MOI) of 100 for 24 h,which was significantly higher than that in hPDLC treated with Pg at MOI of 10∶1 [ (734.09 ± 87.90) ng/L,P =0.000 ],the controls [ (425.8 ± 77.25 ) ng/L,P=0.000] and that in hPDLC treated with Pg at MOI of 100 for 12 h [(1157.50±234.65) ng/L,P=0.000].The expression level of IL-8 reached (4965.81 ± 1072.55 ) ng/L in hPDLC treated with Pg at MOI of 100 for 24 h,which was significantly higher than that in hPDLC treated with Pg at MOI of 10 [ ( 803.51 ±162.08 ) ng/L,P =0.007 ],the controls [ (400.75 ± 2.27 ) ng/L,P =0.005 ] and that in hPDLC treated with Pg at MOI of 100 for 12 h [ ( 1431.12 ± 82.78) ng/L,P =0.001 ].E2 did not show remarkable effect on the expressions of IL-6 and IL-8.E2 combined with Pg ( MOI =100∶1 ) significantly promoted the expression levels of IL-6 at 24 h while did not influence those of IL-8.The relative mRNA level of IL-6 in hPDLC treated with 10-10 mol/L E2 or 10-7 mol/L E2 combined with Pg were 0.49 ± 0.15 (P =0.021 )and 0.53 ±0.16 ( P =0.036 ) individually,which were significantly higher than that treated with Pg alone,0.19 ±0.06.The protein level of IL-6 in hPDLC treated with 10-10 mol/L E2 or 10-7 mol/L E2 combined with Pg were (5512.66 ± 1022.07) ng/L (P =0.012) and (6988.78 ±2279.13) ng/L (P =0.000)individually,which were significantly higher than that treated with Pg alone,(3138.46 ± 183.72) ng/L Conclusions PgW83 significantly increased the expression levels of IL-6 and IL-8 in hPDLC in a dose-and time-dependent manner.Without the infection of periodontal pathogens,estrogen may exert no effect on the expression of IL-6 and IL-8 while it may promote the expression of IL-6 in hPDLC when combined with Pg,which may in turn promote the process of periodontal inflammation.
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