Abstract: Objective To examine the expression of periodontal ligament-associated protein-1(PLAP-1) in the periodontal tissues and periodontal ligament cells(PDLC). Methods The PLAP-1 expression in normal periodontal tissue was examined by immunohistochemistry. The protein expression and mRNA transcription of PLAP-1 in PDLC were investigated by immunocytochemistry and reverse transcription-polymerase chain reaction. Results PLAP-1 was expressed in periodontium but not in cementum, alveolar bone and gingival tissues. PLAP-1 expression was observed in cell plasma, but not in nuclei. There was a 350 bp electrophoresis band representing PLAP-1 mRNA.Conclusions PLAP-1 may play a role in physiology of periodontal tissues and cells in normal adult rats.