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Expression of periodontal ligament-associated protein-1 in normal periodontal tissues and cells in rat

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF STOMATOLOGY
Issue:
6
DOI:
10.3760/cma.j.issn.1002-0098.2011.06.005
Key Word:
牙周组织;牙周膜;动物,实验;牙周膜相关蛋白1;Periodontium;Periodontal ligament;Animals,laboratory;Periodontal ligament-associated protein-1

Abstract: Objective To examine the expression of periodontal ligament-associated protein-1(PLAP-1) in the periodontal tissues and periodontal ligament cells(PDLC). Methods The PLAP-1 expression in normal periodontal tissue was examined by immunohistochemistry. The protein expression and mRNA transcription of PLAP-1 in PDLC were investigated by immunocytochemistry and reverse transcription-polymerase chain reaction. Results PLAP-1 was expressed in periodontium but not in cementum, alveolar bone and gingival tissues. PLAP-1 expression was observed in cell plasma, but not in nuclei. There was a 350 bp electrophoresis band representing PLAP-1 mRNA.Conclusions PLAP-1 may play a role in physiology of periodontal tissues and cells in normal adult rats.

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