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Immunofluorescence examination of CK-13 expression tn cell line KB differentiated by all-trans retinoic acid or As2O3

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF STOMATOLOGY
Issue:
1
DOI:
10.3760/cma.j.issn.1002-0098.2009.01.015
Key Word:
角蛋白13;维甲酸;三氧化二砷;Keratin-13;Tretinoin; Arsenic trioxide

Abstract: Objective To examine the expression of cytokeratin-13(CK-13)in oral squamous cell carcinoma(OSCC)and to discuss the effects of all-trans retinoic acid(ATRA)or arsenic trioxide(As2O3)on the differentiation of human oral undifferentiated squamous cell carcinoma cell line KB cells.Methods The cultured KB cells were divided into three groups,ATRA group,As2O3 group,and control The expression of CK-13 in KB cells was detected using the inmmunofluorescence before and after KB cells were induced by ATRA or As2O3.Results The expression rates of CK-13 in KB cells in the ATRA group and As2O3 group were significantly higher than that in the control(P<0.05),but there was no significant difference in the expression between ATBA and As2O3 group(P>0.05).Conclusions ATRA and As2O3 both have the ability to differentiate the KB cells,and the expression is associated with the degree of tumor differentiation.CK-13 may serve as a molecular marker to evaluate the effect of the differentiation treatment on OSCC.

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