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Immunolocalization of Secretion complex in Streptococcus mutans
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:1002-0098.2007.12.002
- Key Word:
- 链球菌,变异;免疫印迹法;细菌蛋白质类;分泌系统
Abstract： 目的 通过定位SecA和SecY蛋白来定位Sec分泌系统在变形链球菌上的分布形态.方法 用Western blot法检测抗SecA多克隆抗体和SecY多克隆抗体的特异性;胶体金标记免疫电镜观察SecA蛋白和SecY蛋白在变形链球菌GS-5上的分布模式.结果 免疫印迹结果表明,抗SecA蛋白的多克隆抗体和抗SecY蛋白的多克隆抗体可分别特异性地识别相对分子质量为95 000和47 800的抗原.电镜下可见金标记颗粒聚集在变形链球菌GS-5细胞膜上一个微小的区域.结论 蛋白SecA和SecY不对称地聚集在变形链球菌细胞膜上一个微小区域,提示Sec分泌系统在变形链球菌细胞膜上呈现单一位点的分布趋势.
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