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Porphyromonas gingivalis vesicle induced cellular inflammatory responses of gingival epithelial cells in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:1002-0098.2007.09.017
- Key Word:
- 地诺前列酮;环氧化合物;牙龈卟啉单胞菌;体外研究
Abstract： 目的 建立牙龈卟啉单胞菌膜泡诱导牙龈上皮细胞炎性反应的体外模型,探讨牙龈卟啉单胞菌在牙周炎中的致病作用.方法 用酶联免疫吸附法检测牙龈卟啉单胞菌膜泡对牙龈上皮细胞前列腺素E2(prostaglandin E2,PGE2)分泌的影响,以实时反转录聚合酶链反应法检测牙龈卟啉单胞菌膜泡对牙龈上皮细胞环氧化物酶(cyclooxygenase,COX)-2和白细胞介素(interleukin,IL)-6、IL-8基因表达的作用.结果 牙龈卟啉单胞菌膜泡浓度依赖性地促进了牙龈上皮细胞PGE2的分泌,并使COX-2、IL-6、IL-8的mRNA表达水平显著上调.结论 牙龈卟啉单胞菌膜泡诱导牙龈上皮细胞发生的细胞炎性反应,可能是牙周炎发生、发展的重要因素.
- [1]Elisoa, Andrian,Daniel, Grenier,Mahmoud, Rouabhia.In vitro models of tissue penetration and destruction by Porphyromonas gingivalis.[J].2004,72(8).
- [2]N, Kurihara,D, Bertolini,T, Suda,etc.IL-6 stimulates osteoclast-like multinucleated cell formation in long term human marrow cultures by inducing IL-1 release.[J].1990,144(11).
- [3]R P,Darveau.Local chemokine paralysis, a novel pathogenic mechanism for Porphyromonas gingivalis.[J].1998,66.
- [4]Beverly A, Dale.Periodontal epithelium: a newly recognized role in health and disease.[J].2002,30.
- [5]P N, Madianos,P N, Papapanou,J, Sandros.Porphyromonas gingivalis infection of oral epithelium inhibits neutrophil transepithelial migration.[J].1997,65(10).
- [6]T, Njoroge,R J, Genco,H T, Sojar,etc.A role for fimbriae in Porphyromonas gingivalis invasion of oral epithelial cells.[J].1997,65.
- [7]Hiroaki, Inaba,Shinji, Kawai,Takahiro, Kato,etc.Association between epithelial cell death and invasion by microspheres conjugated to Porphyromonas gingivalis vesicles with different types of fimbriae.[J].2006,74(1).
- [8]Madianos PN,Bobetsis YA,Kinane DF.Generation of inflammatory stimuli: how bacteria set up inflammatory responses in the gingiva.[J].2005,32(Suppl.6).
- [9]Haake Susan Kinder,Dang Hoa N,Zhang Hi Bo,etc.Differential regulation of cytokine genes in gingival epithelial cells challenged by Fusobacterium nucleatum and Porphyromonas gingivalis[J].2004,37(6).
- [10]Kato T,Okahashi N,Inaba H,etc.Impaired degradation of matrix collagen in human gingival fibroblasts by the antiepileptic drug phenytoin.[J].2005,76(6).
- [11]Offenbacher Steven,Heasman Peter A.,Collins John G..Modulation of Host PGE2Secretion as a Determinant of Periodontal Disease Expression[J].1993.
- [12]J, Sandros,C, Karlsson,D F, Lappin,etc.Cytokine responses of oral epithelial cells to Porphyromonas gingivalis infection.[J].2000,79(10).