You Position: Home > Paper
Production of interleukin-11 and interleukin-6 in cultured human gingival fibroblasts with the stimulation of lipopolysacchrides
( views:195, downloads:4 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1002-0098.2007.01.011
- Key Word:
- 脂多糖类;白细胞介素11;白细胞介素6;紫单胞菌;龈
Abstract： 目的 观察牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、伴放线放线杆菌(Actinobacillus actinomycetemcomitans,Aa)、大肠杆菌(Escherichia coli,Ec)的脂多糖(lipopolysacchrides,LPS)刺激人牙龈成纤维细胞(human gingival fibroblasts,HGF)合成白细胞介素(interleukin,IL)-11和IL-6的能力,并了解内源性前列腺素是否介导IL-11和IL-6的产生.方法 将3种浓度的LPS(0.1、1、10 mg/L)分别作用于体外培养的HGF 24 h,另外在10 mg/L的LPS中加入10-5 mol/L的吲哚美辛后再分别刺激HGF 24 h,ELISA法检测HGF 上清液中IL-11和IL-6含量的变化.结果 Aa-,Ec-LPS(10 mg/L)和Pg-IPS(1、10 mg/L)刺激HGF后IL-11水平显著提高;Pg-,Ec-LPS(0.1、1、10 mg/L)和Aa-LPS(1、10 mg/L)刺激HGF产生IL-6的水平明显增高;这些作用均受到吲哚美辛的抑制作用.结论 LPS使HGF产生IL-11和IL-6的水平明显增加,并受到内源性前列腺素的正向调控.
- [1]和璐,Nagasawa T,Ishikawa I.IL-1α刺激人牙龈成纤维细胞产生IL-11和IL-6的比较研究[J].2006,(2).
- [2]Li, Li,Alireza, Khansari,Lior, Shapira,etc.Contribution of interleukin-11 and prostaglandin(s) in lipopolysaccharide-induced bone resorption in vivo.[J].2002,70.
- [3]S P, Nair,S, Meghji,M, Wilson,etc.Bacterially induced bone destruction: mechanisms and misconceptions.[J].1996,64(7).
- [4]T, Nakashima,Y, Kobayashi,S, Yamasaki,etc.Protein expression and functional difference of membrane-bound and soluble receptor activator of NF-kappaB ligand: modulation of the expression by osteotropic factors and cytokines.[J].2000,275(3).
- [5]Jagels MA,Fischer WH,Hugli TE.Regulation of IL-6 synthesis in human peripheral blood mononuclear cells by C3a and C3a(desArg).[J].1999,162(1).
- [6]T, Imatani,T, Kato,K, Okuda.Production of inflammatory cytokines by human gingival fibroblasts stimulated by cell-surface preparations of Porphyromonas gingivalis.[J].2001,16(2).
- [7]Noguchi K,Endo H,Shitashige M,etc.Binary regulation of interleukin (IL)-6 production by EP1 and EP2/EP4 subtypes of PGE2 receptors in IL-1beta-stimulated human gingival fibroblasts.[J].2002,37(1).
- [8]K.Noguchi,M.Shitashige,M.Yanai,etc.Prostaglandin production via induction of cyclooxygenase-2 by human gingival fibroblasts stimulated with lipopolysaccharides[J].1996,20(5).
- [9]Tomoko, Kurita-Ochiai,Kuniyasu, Ochiai,Naoto, Suzuki,etc.Human gingival fibroblasts rescue butyric acid-induced T-cell apoptosis.[J].2002,70(5).